IL-6, IL-10 and IL-17 Gene Polymorphisms in Iranian Women with Recurrent Miscarriage
نویسندگان
1 Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran
2 Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz
3 Reproductive Immunology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
4 Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran
5 Nano biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran
6 Immunology Research Center, School of Medicine, Iran University of Medical Sciences, Tehran
7 Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz
doi
10.22034/iji.2014.16770چکیده
Background: Pro-inflammatory and anti-inflammatory cytokines and polymorphisms of their genes have been described to be involved in the pathogenesis of recurrent miscarriage (RM). Objective: To investigate the association between RM and five polymorphisms of cytokine genes, interleukin 10 (IL-10), (-592 A/C, -819 C/T, -1082 A/G), IL-6 (-174 C/G) and IL-17 (-197 G/A) in Iranian women. Method: Polymerase chain reaction -restriction fragment length polymorphism (PCR-RFLP) was performed to determine the frequencies of the IL-6, IL-10 and IL-17 gene polymorphisms in 85 women with RM compared with 104 healthy controls. Results: The frequencies of IL- 10 promoter gene polymorphisms (-592 A/C and -819 C/T) were significantly higher in RM women than those in controls (p=0.003). However, no statistically significant differences were observed in the frequencies of IL-6 (-174 C/G), IL-10 (-1082 A/G) and IL-17 (-197 G/A) polymorphisms between RM women and controls. Conclusion: These results suggest that IL-10 gene polymorphism screening might have some relevance in patients with RM, a suggestion which requires further studies.
