Bone Marrow and Peripheral Blood Mononuclear Cell Phenotype Changes after Cultivation and Autologous Infusion in Patients with Primary Biliary Cholangitis
نویسندگان
1 Cell Technologies, Transplantation and Research Department, National Scientific Medical Center, Astana, Kazakhstan
2 PerciaVista R&D Co. Shiraz, Iran
3 Department of Urology and Andrology, Astana Medical University, Astana, Kazakhstan
4 Clinical and Diagnostic Laboratory, National Scientific Medical Center, Astana, Kazakhstan
5 Surgical Diseases, Angiosurgery and Plastic Surgery Department, Astana Medical University, Astana, Kazakhstan
6 General Surgery, West Kazakhstan Marat Ospanov Medical University, Aktobe, Kazakhstan
7 General Surgery, West Kazakhstan Marat Ospanov Medical University, Aktobe, Kazakhstan
8 Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
9 Department of Anatomy, School of Biomedical Sciences, Medicine and Health, UNSW Sydney, Sydney, Australia
10 Cell Technologies, Transplantation and Research Department, National Scientific Medical Center, Astana, Kazakhstan
11 Sheffield Institute for Translational Neuroscience (SITraN), Department of Neuroscience, University of Sheffield, 385 Glossop Road, Sheffield S10 2HQ, UK
12 Clinical and Diagnostic Laboratory, National Scientific Medical Center, Astana, Kazakhstan
13 Department of Surgery, National Scientific Medical Center, Astana, Kazakhstan
14 Surgical Diseases, Angiosurgery and Plastic Surgery Department, Astana Medical University, Astana, Kazakhstan
15 General Surgery, West Kazakhstan Marat Ospanov Medical University, Aktobe, Kazakhstan
16 Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
17 Department of Applied Cell Sciences, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
18 Reproductive Development, Murdoch Children’s Research Institute, Melbourne, Victoria, Australia
19 PerciaVista R&D Co. Shiraz, Iran
doi
10.30476/ijms.2023.99613.3172چکیده
Background: Primary biliary cholangitis (PBC) is a condition affecting the liver and immune system. In this study, the impact of autologous bone marrow-derived mononuclear cell (BM-MNC) transplantation on PBC patients was investigated. Methods: Sixteen eligible PBC patients participated at the National Scientific Medical Center in Astana, Kazakhstan, between 2017 and 2022, and BM-MNCs were harvested from their anterior iliac crest. After isolating and cultivating the BM-MNCs, they were infused back into the patient’s peripheral veins. Changes in BM-MNC and peripheral blood mononuclear cell (PB-MNC) phenotypes were assessed before and after a 24-hour cultivation period and 72 hours post-transplantation. We monitored liver function parameters over 6-month intervals and conducted flow cytometry analysis to assess CD markers on BM-MNCs before and after cultivation and PB-MNCs before and after transplantation. Statistical analysis included the Friedman test for liver parameters and the Wilcoxon signed-rank test for BM-MNC and PB-MNC comparisons.Results: Our findings revealed significant reductions in liver function tests after multiple transplantations. Flow cytometry analysis before and after a 24-hour culture and autologous BM-MNC infusion revealed the expansion of specific cell populations, with significant increases in CD3+, CD4+, CD16+, CD20+, CD25+, CD34+, CD105+, CD73+, СD117+, and CD34+populations, while CD4+25+, CD34+105+, and CD4+FOXP3+ populations decreased. Interestingly, a contradictory finding was observed with a decrease in bone marrow CD34+105+ cell lines (P=0.03) alongside an increase in peripheral CD34+105+ population (P=0.03).Conclusion: In summary, our study shows that BM-MNC transplantation in PBC patients leads to changes in immune cell populations and liver function. These findings suggest potential therapeutic applications of BM-MNC transplantation in managing PBC and offer insights into the dynamics of immune cells associated with this treatment approach.
