Effect of Substrate Concentration on Simultaneous Acetone-Butanol-Ethanol Fermentation and Biohydrogen Production from Fig (Ficus carica)
نویسندگان
1 Department of Biotechnology, Graduate School of Natural and Applied Sciences, Dokuz Eylul University P. O. Box: 35390, İzmir, Türkiye.
2 Department of Biotechnology, Graduate School of Natural and Applied Sciences, Dokuz Eylul University P. O. Box: 35390, İzmir, Türkiye.
3 Department of Environmental Engineering, Faculty of Engineering, Dokuz Eylul University, P. O. Box: 35390, İzmir, Türkiye.
4 Department of Microbiology, College of Biosciences, Federal University of Agriculture, P. O. Box: 110111, Abeokuta, Nigeria.
5 Department of Biotechnology, Graduate School of Natural and Applied Sciences, Dokuz Eylul University P. O. Box: 35390, İzmir, Türkiye.
6 Institute of Sustainable Energy, Universiti Tenaga Nasional, P. O. Box: 43000, Kajang, Malaysia.
7 Department of Biological Sciences, College of Natural and Applied Sciences, Crescent University, P. O. Box: 110001, Abeokuta, Nigeria.
doi
10.30501/jree.2025.511732.2303چکیده
The growing demand for renewable and sustainable fuel alternatives has driven extensive research into biohydrogen production and acetone-butanol-ethanol (ABE) fermentation, with butanol as the primary product of interest. To address the fuel-versus-food dilemma, this study explores the biofuel potential of lignocellulosic biomass, specifically fig (Ficus carica), building upon our previous findings. Substrate concentration plays a pivotal role in fermentation outcomes, either enhancing or inhibiting product yields. This study investigates the effects of varying substrate concentrations (20–47 g L⁻¹) on ABE fermentation and biohydrogen production from fig hydrolysate. Results demonstrated that higher substrate concentrations significantly improved product yields: the maximum cumulative hydrogen production (1402 mL) and highest solvent concentrations (ethanol: 1.45 g L⁻¹; acetone: 8.08 g L⁻¹; butanol: 5.09 g L⁻¹) were achieved at 47 g L⁻¹. The biphasic ABE fermentation process yielded peak organic acid production (acetic acid: 10.49 g L⁻¹; butyric acid: 6.02 g L⁻¹) at 47 g L⁻¹ and 39 g L⁻¹, respectively. These findings underscore the positive correlation between increased substrate concentration and enhanced ABE fermentation efficiency, highlighting the potential of fig as a sustainable feedstock for biofuel production.
