فهرست نشریات علمی دسترسی آزاد ایران

Background: Lung cancer is associated with the activation of inflammatory pathways and increased levels of the pro-inflammatory cytokine tumor necrosis factor-alpha (TNF-α). The present study aimed to investigate the effects of swimming training and royal jelly supplementation on TNF-α gene expression in the lung tissue of an animal model of lung cancer. Methods: In this experimental study, 48 male Balb/C mice, aged eight weeks, were divided into the following groups: healthy control, sham, lung cancer (BZP), swimming exercise (ST), royal jelly 50 mg/kg (RJ50), royal jelly 100 mg/kg (RJ100), swimming + royal jelly 50 mg/kg (ST.RJ50), and swimming + royal jelly 100 mg/kg (ST.RJ100). Lung cancer was induced via intraperitoneal injection of 100 mg/kg benzo[a]pyrene. Swimming training was performed 3days per week for 12 weeks, and royal jelly was administered intraperitoneally at doses of 50 and 100 mg/kg. 48hours after the last exercise session, animals were sacrificed, lung tissues were collected and frozen, and TNF-α gene expression was measured using real-time PCR. Results: TNF-α levels were significantly elevated in the BZP, RJ50, RJ100, and ST groups compared to the healthy control and sham groups. TNF-α expression in the ST.RJ50 group was also significantly higher than the healthy control. In the intervention groups (ST, ST.RJ50, and ST.RJ100), TNF-α expression was significantly lower than in the BZP group. Moreover, TNF-α expression in the ST.RJ50 and ST.RJ100 groups was significantly lower than in the RJ50 and RJ100 groups. Conclusion: Swimming training, especially when combined with royal jelly, can reduce TNF-α expression in the lung tissue of Mice with benzo[a]pyrene-induced lung cancer. This combination may serve as a complementary anti-inflammatory and preventive strategy to control the progression of lung cancer.