Nettle (Urtica Dioica L.) Modulates Angiogenesis by Targeting the PI3K/AKT/eNOS Pathway in Prostate Cancer
نویسندگان
1 College of Medicine, University of Thi-Qar, Al-Nasiriyah, Iraq
2 Department of Medical Laboratory Techniques, Al-Safwa University College, Karbala, Iraq
3 Prosthetic Dental Techniques Department, Al-Mustaqbal, University College, Hillah, Babylon, Iraq
4 Department of Biochemistry, College of Medicine, University of Anbar, Al-Anbar Governorate, Ramadi City, Iraq
5 Department of Pharmaceutical Chemistry, College of Pharmacy, University of Mosul, Mosul, Iraq
6 Department of Medical Laboratory Techniques, Al-Maarif University College, Al-Anbar Governorate, Ramadi City, Iraq
7 Desert Studies Center, University of Anbar, Al-Anbar, Iraq
doi
10.30476/mejc.2024.102467.2086چکیده
Background: Medicinal plants, predominantly those rich in polyphenolic mixtures, have been recommended to have chemopreventive and chemotherapeutic effects. This study aimed to investigate the effects of Urtica dioica L. extract (UDE) on angiogenesis and prostate cancer (PCa) cell progression through the phosphoinositide 3-kinase/Protein kinase B/ endothelial nitric oxide synthase (PI3K/AKT/eNOS) signaling pathway.Method: This study employed an in vitro experimental design using PCa cell lines. To gain mechanistic insights into the anti-proliferative properties of UDE, PCa cell proliferation was assessed using an MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay in DU-145 cells (incubated for 48h). Also, we explored expression patterns of PI3K/AKT/eNOS pathway genes with therapeutic potential (with 50 µg/ml of UDE) in DU-145 cells by quantitative polymerase chain reaction and western blotting assay. Furthermore, we applied the ELISA cell death assay kit to reveal the apoptotic effects of UDE on PCa cells. Statistical analysis was determined using the Mann-Whitney U test and P ≤ 0.05 was considered statistically significant. Results: UDE significantly decreased the cell viability after 48 h of treatment in DU-145 cells. Also, reducing the vascular endothelial growth factor (VEGF) levels revealed anti-angiogenic outcomes. Also, the eNOS level in the PI3K/AKT/eNOS pathway is dramatically alleviated upon treatment with UDE. Moreover, the apoptosis rate of DU-145 cells was enhanced compared with the control group.Conclusion: The antitumoral activity of UDE was prominent in its persuasive anti-angiogenic potential, as UDE contributed to a striking diminish in PI3K/AKT/eNOS pathway in PCa and diminished the VEGF expression.
