Antibacterial and Anti-biofilm Investigation of Electrospun PCL/gelatin/Lawsone Nano Fiber Scaffolds against Biofilm Producing Bacteria
نویسندگان
1 Textile Engineering Department, Amirkabir University of Technology, Tehran, Iran
2 Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran
3 Department of Biotechnology, Institute of Sciences, High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran
4 Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran
doi
چکیده
The aim of this study was to evaluate anti bacterial and antibiofilm activity of PCL/gelatin nanofibers containing Lawsone against four pathogenic bacteria. Different concentrations of Lawsone (2-hydroxy-1, 4- naphthoquinone) (1, 3, 5 and 10%) were incorporated into polycaprolactone (PCL), gelatin (GEL) nanofibers via electrospinning technique. Presence of Lawsone in the scaffold confirmed by scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR). Antibacterial and antibiofilm activity of Lawsone loaded scaffolds against clinically isolated Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa and Proteus mirabilis were investigated by disc diffusion method. All scaffolds were able to inhibit the growth of S. aureus and MRSA. Additionally, PCL/GEL/Lawsone 10% had bactericidal efficacy against P. mirabilis. Obtained results of the biofilm formation assay revealed that PCL/GEL/Lawsone 10% inhibited the biofilm of S. aureus, MRSA and P. mirabilis by 56%, 32%, and 15%, respectively. Due to good antibacterial and antibiofilm activity of Lawsone containing scaffolds, PCL/GEL 10% can be a good candidate for application in wound dressing patches.
