Evaluation of Curcumin Nano-micelle on Proliferation and Apoptosis of HT29 and Hct116 Colon Cancer Cell Lines
نویسندگان
1 Pharmaceutical Sciences Research Center, Mazandaran University of Medical Sciences, Sari Iran
2 Pharmaceutical Sciences Research Center, Mazandaran University of Medical Sciences, Sari Iran
3 Pharmaceutical Sciences Research Center, Mazandaran University of Medical Sciences, Sari Iran
4 Pharmaceutical Sciences Research Center, Mazandaran University of Medical Sciences, Sari Iran
5 Pharmaceutical Sciences Research Center, Mazandaran University of Medical Sciences, Sari Iran
6 Pharmaceutical Sciences Research Center, Mazandaran University of Medical Sciences, Sari Iran
7 Pharmaceutical Sciences Research Center, Mazandaran University of Medical Sciences, Sari Iran
8 Department of Pharmacology, School of Medicine, Iran University of Medical Sciences, Tehran Iran
doi
10.30476/mejc.2021.87699.1429چکیده
Background: Curcumin is a natural polyphenolic material with antioxidative, anti-inflammatory, and anticancer effects. In this study, we attempted to assay antiproliferative and apoptotic properties of polymeric micelles of curcumin on two colorectal cancer cell lines and normal human fibroblast cells.Method: In this experimental study, cancer cells HT29, HCT116 and normal human fibroblast cells (HGF) were subjected to concentrations of Nano- curcumin (1, 50, 100, 250, and 500 μg/ml). After incubation for 48 hours, cell viability was assessed with "MTT"(3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay. Annexin V-FITC and Propidium iodide staining were done with flow cytometry for evaluation of apoptosis. The results were shown as mean ± standard deviation. Statistical significance was assessed utilizing ANOVA and Dunnetts t-test (P < 0.01).Results: According to MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay results, IC50 value of Nano- curcumin in HT29, HCT116, and HGF were 70.63, 123.9, and 168.53 μg/ml, respectively. We also discovered that Nano-curcumin can make indicative apoptosis in cancer cells, which could be compared with cisplatin <0.01.Conclusion: These results revealed remarkable antiproliferative and apoptotic effects of polymeric Nano-micelles of curcumin in colorectal cancer cell lines.
