Molecular Diagnosis of Tuberculosis: a New Primer Design
نویسندگان
1
2 دانشگاه علوم پزشکی تبریز
3 دانشگاه علوم پزشکی تبریز
4
doi
چکیده
Background: Nucleic acid based detection of Mycobacterium tuberculosis has the potential to improve the analysis of the tuberculosis epidemiology and patient care by increasing the specificity and sensitivity of diagnosis. Results: The mean age of cases was 33.6±14.3 years. Twenty one cases (42%) were positive for M. tuberculosis. Maximum positivity was found in sputum (10/19) followed by menstrual blood (4/8), blood (5/15), ascitic fluid (1/2), and pleural fluid (1/5). Conclusion: The new designed primer was reproducible and in complete concordance with the PCR-results obtained by the standard method using IS 6110 insertion sequence in all 50 cases in which both methods were used.