فهرست نشریات علمی دسترسی آزاد ایران

Background: Infections caused by Shigella are a major cause of diarrheal disease in the developing and developed countries. The present study was conducted to apply and evaluate arbitrarily primed PCR (AP-PCR) for investigation of genetic relatedness among the strains of Shigella sonnei isolated from cases of acute diarrhea in Tehran. Results: All strains of S. sonnei were typeable with this approach. APPCR generated nine indistinguishable bands ranged from 0.35 to 2.5 kbp in all studied strains. Only a single AP-PCR pattern was observed among the S. sonnei strains recovered in 2003. Two sporadic isolates recovered in 1984 showed different AP-PCR patterns compared to recent clinical isolates. Conclusion: Results suggest that a very homogeneous AP-PCR cluster types might be responsible for shigellosis caused by S. sonnei in Tehran in 2003. Further molecular analysis conducted on a larger selection of isolates could confirm our findings.