فهرست نشریات علمی دسترسی آزاد ایران

Background: Neisseria meningitidis is a major cause of bacterial septicemia and meningitis. Currently, there are no vaccines to prevent disease caused by strains of N. meningitidis serogroup B, since cross-reactivity of the serogroup B capsule with human tissue has hampered efforts to develop a reliable vaccine. PilQ is an antigenically conserved outer membrane protein which is essential for meningococcal pilus expression at the cell surface. Results: Our results show pilQ406-770 cloned in pET28a vector, while the cloning of pilQ406-770 was confirmed by colony- PCR and enzymatic digestion. SDS-PAGE analysis showed that our constructed prokaryotic expression system pET28apilQ406- 770-BL21efficiently produces target recombinant protein with molecular weight of 43 kDa in the form of dissoluble inclusion body. Conclusion: Our results confirmed that a prokaryotic expression system for PilQ406-770 protein was successfully constructed.