فهرست نشریات علمی دسترسی آزاد ایران

Objective: Brucellosis is a zoonotic disease of which diagnosis is based on clinical symptoms and positive laboratory findings. Since serology tests are not specific and sensitive enough, polymerase chain reaction (PCR) can be an alternative method in making the final decision in suspicious cases. In this study, three diagnostic methods were compared in suspected patients with brucellosis in the endemic area of Mianeh, Iran. Results: We found 28 cases with positive results for B. melitensis by Multiplex PCR technique which was significantly different from of SAT (P<0.05). Six samples were positive for B. abortus by PCR. Conclusion: The results of present study showed that Multiplex PCR assay is a rapid and sensitive technique for diagnosis of brucellosis compared to SAT. However it is more accurate when coupled with conventional methods.