فهرست نشریات علمی دسترسی آزاد ایران

Background: Listeria monocytogenes , as one of the foodborne pathogens, is a causative agent of listeriosis. The transfer of L. monocytogenes bacteremia in pregnant women occurs as self-limited flu-like symptoms, but it may result in abortion, stillbirth or premature birth of the infected baby. One of the best methods for detection of this bacterium is polymerase chain reaction (PCR). Objectives: The purpose of this study was detection of virulence factors (hlyA and plcA) of L. monocytogenes in women with abortion, using PCR. Results: Out of 96 samples, 16 isolates of L. monocytogenes by PCR (plcA and hlyA) and four isolates by culture were identified. There was a significant difference between PCR and culture methods (P = 0.003). The results of this study showed that PCR was more sensitive and specific than culture method. There was also a significant association between the bacteria and hlyA and plcA genes and human abortion and between patients with abortion precedence and education. Conclusions: Based on our study, plcA and hlyA played a key role in the virulence determination of L. monocytogenes . Data analysis also showed that L. monocytogenes could be a causative agent of abortion in pregnant women.