Histomorphometric effects of gemcitabine on Swiss albino mice spermatogenesis
نویسندگان
1 Department of Anatomy, A.J. Institute of Medical Sciences, Mangalore, Karnataka, India
2 Department of Pharmacology, Azeezia Institute of Medical Sciences, Meeyannoor, Kollam, Kerala, India
3 Department of Anatomy, Azeezia Institute of Medical Sciences, Meeyannoor, Kollam, Kerala, India
4 Department of Anatomy, Amala Institute of Medical Sciences, Thrissur, Kerala, India
doi
چکیده
Background: Spermatogenesis is a highly conserved and regulated process and it is sensitive to fluctuations in the physical and chemical environment. Gemcitabine is a novel antimetabolic anticancer drug used frequently in the treatment of many cancers. This study aimed to investigate the histomorphometric effects of gemcitabine on spermatogenesis in Swiss albino mice.Materials and Methods: Gemcitabine in high and low doses (80 and 40 mg/kg) injected intraperitoneally to inbred Swiss albino mice. Gross testicular features and seminiferous tubular histomorphometry was studies at the end of 7 th , 14 th day and at 2 months sperm shape abnormalities were studied.Results: Seminiferous tubular morphology was altered significantly, showing a reduction in height, perimeter and area in a dose dependent manner. Sertoli cell number decreased. Basement membrane thickness was reduced and it appeared to be permanent, with statistically insignificant changes even after 2 months. There was a reduction of intertubular spaces. Sperms have shown banana heading, decapitation and loss of normal hook of head. The effects were partially reversible at the end of 2 months.Conclusion: It was concluded that gemcitabine affects the process of spermatogenesis adversely in a dose and time dependent manner and the effects are partially reversible.
