فهرست نشریات علمی دسترسی آزاد ایران

Objective: Breast cancer remains one of the leading causes of mortality among women worldwide. Approximately80% of patients receive radiotherapy (RT) as part of their treatment. Numerous efforts have been made to distinguishradiosensitive from radioresistant breast tumors. Long non-coding RNAs (lncRNAs) have increasingly been recognizedas crucial mediators of cellular responses to radiation among the wide range of biomarkers studied. This study exploredthe radiation-induced expression changes of three apoptosis-associated lncRNAs—MEG3, MALAT1, and ANRIL—inbreast cancer.Materials and Methods: In this experimental study, two established human breast cancer cell lines, MCF-7 and MDAMB-231, were cultured in Dulbecco’s Modified Eagle Medium (DMEM) and irradiated with X-rays at doses ranging from0 to 8 Gy. The expression levels of the lncRNAs ANRIL, MALAT1 and MEG3 were quantified by real-time polymerasechain reaction (PCR). Cell viability in irradiated MCF-7 and MDA-MB-231 cells was evaluated using the MTT colorimetricassay.Results: Following radiation exposure, the expression levels of ANRIL and MEG3 were significantly decreased (P=0.004)in both MCF-7 and MDA-MB-231 cell lines, whereas MALAT1 expression was significantly increased (P=0.006) only inMDA-MB-231 cells. In addition, cell viability in both MCF-7 and MDA-MB-231 lines declined after irradiation.Conclusion: Our findings indicate that radiation exposure increases MALAT1 expression while decreasing ANRILand MEG3 levels, associated with reduced cell viability. These results suggest a potential role for these lncRNAs inradiation-induced cell death.