Mac-1 Alongside Platelet-Monocyte Aggregates as Potential Markers in Acute Coronary Syndrome: A Case-Control Study
نویسندگان
1 Department of Hematology and Blood Banking, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2 Department of Cardiology, Taleghani General Hospital, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3 Department of Hematology and Blood Banking, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4 Research Center for Social Determinants of Health, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5 Department of Biostatistics, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
6 Department of Laboratory Sciences, School of Paramedicine, Shahid Saduoghi University of Medical Sciences, Yazd, Iran
7 Department of Hematology and Blood Banking, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
8 Department of Medical Laboratory Sciences, Faculty of Allied Medicine, Gonabad University of Medical Sciences, Gonabad, Iran
9 Department of Cardiology, Taleghani General Hospital, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
10 Department of Hematology, Faculty of Allied Medical Sciences, Zabol University of Medical Sciences, Zabol, Iran
doi
10.22074/cellj.2024.2024525.1527چکیده
Objective: Cardiovascular diseases (CVDs) are the leading cause of death worldwide, with atherosclerosis servingas a primary factor in their development. Platelets, leukocytes, and their interactions play a crucial role ininitiating and amplifying atherosclerosis. This study aims to evaluate the levels of platelet-monocyte aggregates (PMA)and specific integrins involved in leukocyte recruitment, including macrophage-1 antigen (Mac-1) and lymphocytefunction-associated antigen-1 (Lfa-1), in patients with acute coronary syndrome (ACS).Materials and Methods: In this case-control study, thirty-two subjects with ACS and 30 healthy individuals participated.It aimed to evaluate PMA expression and the median fluorescence intensity (MFI) of Mac-1 and Lfa-1 using flowcytometry. Dot plots and Pearson correlation coefficient were employed to examine the relationship between PMA,Mac-1, and Lfa-1. Multilevel model analysis was used to explore the effects and relationships of various parameters,including Mac-1 and Lfa-1, on PMA. Finally, receiver operating characteristic (ROC) curves were utilized to assessthe diagnostic accuracy of PMA, Mac-1, and Lfa-1 markers.Results: It was observed that patients had higher PMA levels compared to the control group (58.99 ± 16.27 vs.29.99 ± 4.19 in controls, P<0.001), which correlated with PLT (ρ=0.512, P=0.035). Additionally, CD18 and CD11bexpression on monocytes were significantly elevated in patients (P<0.001) and were positively associated with PMA(β=19.09, P<0.001; β=6.90, P=0.022), but no significant relationship between CD11a and PMA was observed (β=5.06,P=0.315). PMA and Mac-1 were identified as better markers for differentiating patients from healthy individuals.(respectively, AUC=0.94, Sensitivity= 0.84, specificity=0.98; AUC=0.84, Sensitivity= 0.93, specificity=0.70).Conclusion: The study results indicated an increase in both Mac-1 and PMA levels in patients with ACS. Additionally,the significant association observed between Mac-1 and PMA in the patient group suggests a potential relationshipbetween these markers and ACS.
