The Immunomodulatory Aspect of Quercetin Penta Acetate on Th17 Cells Proliferation and Gene Expression in Multiple Sclerosis
نویسندگان
1 Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2 Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
3 Iranian Multiple Sclerosis and Neuroimmunology Research Center, Isfahan, Iran
4 Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
5 Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
6 Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
7 Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
8 Department of Neurology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
doi
10.22074/cellj.2022.557560.1073چکیده
Objective: The function of Th17 cells in the neuroinflammatory process in multiple sclerosis (MS) has been previouslyclarified. It has been suggested that Quercetin can influence MS due to a variety of anti-inflammatory effects. The presentstudy aimed to examine in vitro immunomodulatory aspects of Quercetin Penta Acetate as a modified compound onTh17 cells of MS patients and also to compare its effects with Quercetin.Materials and Methods: In this experimental study, peripheral blood mononuclear cell (PBMCs) were isolated andstained with CFSE then, half-maximal inhibitory concentration (IC50) values were determined using different dosesand times for Quercetin Penta Acetate, and Methyl Prednisolone Acetate. Th17 cell proliferation was analyzed by flowcytometry and the expression levels of IL-17 and RORc genes were assessed by real-time polymerase chain reaction(PCR) method.Results: The results showed that IL-17A gene expression was inhibited by Quercetin Penta Acetate (P=0.0081), butQuercetin Penta Acetate did not have a significant inhibitory effect on Th17 cells proliferation (P= 0.59) and RORc geneexpression (P=0.1), compared to Quercetin.Conclusion: Taken together, our results showed some immunomodulatory aspects of Quercetin Penta Acetate onTh17 cells are more effective than Quercetin and it could be considered in the treatment of MS.
