Leaf, Seed and Pollen Micromorphology in Datura innoxia and Datura stramonium: Implications for Taxonomy and Species Delimitation

نویسندگان

1 Department of Plant Sciences, Faculty of Biological Sciences, Alzahra University

2 Department of Botany, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

3 Dept. Plant Sciences, Faculty of Biological Sciences, Alzahra University, Tehran, Iran

doi
10.48308/pae.2026.242023.1125
چکیده

The genus Datura (Solanaceae) comprises medicinal and toxic plants with a wide global distribution, two of which are native to Iran. In the present study, populations of D. innoxia and D. stramonium were collected from different regions of Iran. Microscopic and micromorphological traits were examined using light microscopy and scanning electron microscopy. Quantitative and qualitative data were analyzed by multivariate statistical methods, to determine the differential value of micromorphological traits of leaves, seeds, and pollen grains to better distinguish Datura species in Iran. The results revealed that leaf surface features, as well as seed traits and pollen morphology provided clear distinctions between the two species. Datura innoxia exhibited dense leaf trichomes, smoother seed surfaces, and striato-reticulate pollen ornamentation, whereas D. stramonium had nearly glabrous leaves, rougher seed surfaces, and rugulate-reticulate pollen ornamentation. The type, length and density of epidermal trichomes, exine ornamentation, lumen type, muri arrangement, exine thickness, length of pollen axes and seed shape and size were of diagnostic importance. The findings demonstrate that micromorphological traits of leaves, seeds, and pollen are of high taxonomic value in distinguishing Datura species in Iran and can serve as complementary tools in systematic studies and identification keys. Nevertheless, research gaps remain, including the need for more extensive population sampling, precise quantitative measurements, statistical comparisons across different geographical regions, and integration of micromorphological data with molecular phylogeny.