فهرست نشریات علمی دسترسی آزاد ایران

Calotropis procera, a medicinally and industrially valuable plant of the Apocynaceae family, faces challenges in propagation due to declining seed germination potential and lack of set mass production methods. This study evaluates the effects of plant growth regulators, specifically 2,4-Dichlorophenoxyacetic acidichlorophenoxyacetic acid and Thidiazuron, on callus induction, shoot formation, leaf development, and root induction in stem explants of C. procera. Tissue culture techniques were employed to address propagation challenges and conserve this species.The highest callus induction (100%) was achieved using 7.5 mg/L 2,4-Dichlorophenoxyacetic acidichlorophenoxyacetic acid and 7.5 mg/L Thidiazuron, with auxins and cytokinins demonstrating a synergistic effect in promoting cell division. For shoot formation, the optimal combination was 1.25 mg/L 2,4-Dichlorophenoxyacetic acidichlorophenoxyacetic acid and 5 mg/L Thidiazuron, while leaf formation peaked with 1.25 mg/L Thidiazuron alone. Excessive Thidiazuron concentrations, however, inhibited leaf formation, underscoring the importance of hormonal balance. Root induction was most effective with 2.5 mg/L 2,4-Dichlorophenoxyacetic acidichlorophenoxyacetic acid and 7.5 mg/L Thidiazuron, whereas treatments with high cytokinin concentrations were found to hinder root growth.These findings align with prior research on other medicinal plants, such as Catharanthus roseus and Calotropis gigantea, demonstrating the complementary roles of auxins and cytokinins in plant tissue culture. This study highlights the potential of tissue culture as a scalable and sustainable method for the propagation and conservation of C. procera, ensuring the preservation of its medicinal and industrial applications.This study confirmed that the proper balance between auxin and cytokinin is important for the successful induction of each growth phase in the plant. This study highlights the potential of tissue culture as a scalable and sustainable method for the propagation and conservation of C. procera, ensuring the preservation of its medicinal and industrial applications.