Detection Of Nitric Oxide Reduction During Ischaemia-Reperfusion By EPR Spectroscopy

نویسندگان
Kadkhodaei, M. ID 1
Willgoss, D.A. ID 2
Gobe, G.C. ID 3
Endre, Z.H. ID 4
Zhang, B. ID 5
Hanson, G.R. ID 6

1 PHYSIOLOGY DEPARTMENT, TEHRAN MEDICAL SCIENCES UNIVERSITY, TEHRAN, IRAN

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چکیده

Introduction: Acute renal failure is a common consequence of sepisis due to concurrent renal ischaemia. The role of nitric oxide (NO) in endotoxaemia and in ischaemic injury in the kidney is not well defined.Material and Methods: In this study we have used an animal model of sepsis induced by injection of bacterial lipopolysaccharide (LPS) in the rat and measured renal nitric oxide by X-band electron paramagnetic resonance (EPR) spectroscopy using the spin trap Fe2+-N-methyl-D-glucamine dithiocarbamate [Fe(MGD)2] given by intravenous injection 6 minutes before sacrifice.Results: The characteristic EPR spectrum of [Fe(NO)(MGD)2] was observed in kidneys of rats treated with LPS for 5h. Rat kidneys subjected to 20 min ischaemia and 5 min reperfusion had lower concentrations of [Fe(NO)(MGD)2] (1.0 ± 0.6 (M) compared to the contralateral nonischaemic kidneys (1.5 ± 0.9 (M, P<0.05).Conclusion: This study shows reduced levels of NO after renal ischaemia in vivo.

کلیدواژه‌ها
Nitric Oxide, ELECTRON PARAMAGNETIC RESONANCE, Kidney, ISCHAEMIA. REPERFUSION