Molecular epidemiology of clinically relevant single and mixed species in a Malaysian tertiary care hospital
نویسندگان
1 Shaukat Khanum Memorial Cancer Hospital and Research Centre, Lahore, Pakistan
2 Faculty of Medicine, Manipal University College, Melaka, Malaysia
3 Department of Biochemistry, Faculty of Medicine, Bioscience, and Nursing MAHASA University; Kuala Lumpur, Malaysia
4 Department of Medical Microbiology, Faculty of Medicine, AIMST University, Malaysia
5 Department of Microbiology, Army Medical College, Jashore, Bangladesh
6 Department of Periodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Chennai, India.
7 Pathology Department, Hospital Sultan Abdul Halim, Ministry of Health Malaysia, Kedah, Malaysia.
doi
10.22034/cmm.2023.345084.1432چکیده
Background and purpose: The increasing rate of opportunistic infections caused by Candida and other yeasts is becoming a major health concern worldwide. However,systematic data on the epidemiology and the yeast species infections in Malaysia is still limited. In this regard, the present research aimed to identify pathogenic yeasts utilizing an economically practical and easily available molecular technique and evaluate the prevalence of pathogenic yeasts in a Malaysian tertiary care hospital.Materials and Methods: Yeast isolates were collected from Sultan Abdul Halim Hospital, Kedah, Malaysia, from October 2020 to October 2021. Molecular identification of the isolates was performed by one enzyme-based polymerase chain reaction restriction fragment length polymorphism method.Results: Candida albicans was the most prevalent species, accounting for 120 isolates (59%) in total. The most prevalent non albicans Candida species were C. tropicalis (n=33,16%), C. krusei (Pichia kudriavzevii) (n=12, 5.8%), C. glabrata (n=12, 5.8%), and C.parapsilosis (n=6, 3%). Other unusual Candida species were C. guilliermondii (2), C.metapsilosis (2), C. orthopsilosis (1), C. lusitaniae (1), C. rugosa (1), C. haemulonii (1), C. bracarensis (1), and C. dubliniensis (1). Moreover, Talaromyces marneffei (1), Kodamaea ohmeri (1), Cryptococcus neoformans (3), and Cryptococcus laurentii (1) were among the other yeasts identified.Conclusion: The Molecular technique used in this study identified 96% of isolates,including mixed species. According to the findings, the most prevalent species are C. albicans, C. tropicalis, C. krusei, and C. glabrata.
