فهرست نشریات علمی دسترسی آزاد ایران

Background: The alarming rise of resistant microorganisms is becoming a serious health threat today. Consequently, there is a pressing need to obtain novel compounds with antimicrobial properties. In this regard, lichens are promising substances with antimicrobial potential to control different microbial strains, including fungi, Gram-positive and Gram-negative bacteria, and viruses. Objectives: This study explored the efficacy of Dermatocarpon miniatum in treating burn wound infections. We examined twenty-four Methicillin-resistant Staphylococcus aureus (MRSA) isolated from patients admitted to Imam Moussa Kazem Hospital of Isfahan, Iran. Methods: In this interventional study, we assessed the antibacterial activity of D . miniatum against MRSA using the microdilution broth method. Biofilm inhibitory was evaluated through microtiter plate technique. Additionally, the effect of D . miniatum on the icaA gene expression was analyzed by real-time PCR. Results: The methanolic extract of D . miniatum exhibited significant antibacterial activity against MRSA strains. Additionally, at a concentration of ½ MIC, the extract demonstrated a 65% inhibition of biofilm formation. Moreover, a sub-MIC concentration (1/4) of D . miniatum was effective in reducing the expression of the icaA gene. Conclusion: The results of the present study reveal that D. miniatum extract can be used as a promising antibacterial agent against MRSA strains commonly found in wound biofilms.