فهرست نشریات علمی دسترسی آزاد ایران

Background: Vitamin C is well recognized for its role in collagen synthesis and wound repair; however, its effects on critical healing parameters warrant further investigation. This study aimed to evaluate the impact of vitamin C on fibroblast count, collagen density, and epithelial thickness in Wistar rats with incisional wounds.Methods: This experimental study investigated the effects of vitamin C on wound healing in Wistar rats with incisional wounds. The rats were divided into a control group and a treatment group, with the latter receiving daily subcutaneous injections of vitamin C (nine mg) for seven days. Histopathological analyses were performed on skin biopsies from the rats' backs using Hematoxylin-Eosin and Masson’s trichrome staining to assess fibroblast count, collagen density, and epithelial thickness. Data were analyzed using SPSS version 24.0, employing Mann-Whitney U tests for fibroblast counts and independent t-tests for collagen density and epithelial thickness. Pearson’s correlation coefficient was used to assess the relationship between fibroblast count and collagen density.Results: Vitamin C treatment significantly increased fibroblast count by 28.08% (P = 0.018), collagen density by 25.75% (P = 0.013), and epithelial thickness by 34.07% (P = 0.001) compared to the control group. No significant correlation (P = 0.125) was observed between fibroblast count and collagen density.Conclusion: Vitamin C can increase the number of fibroblasts, collagen density, and epithelial thickness in incision wounds in Wistar rats. These findings suggest that vitamin C may enhance wound healing outcomes; however, further research is necessary to explore the complex interactions among these factors.