In vitro antitumor activity of patulin on cervical and colorectal cancer cell lines
نویسندگان
1 Immunogenetics Research Center, Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran
2 Department of Medical Mycology and Parasitology, Invasive Fungi Research Center (IFRC), Mazandaran University of Medical Sciences, Sari, Iran
3 Department of Medical Mycology and Parasitology, Invasive Fungi Research Center (IFRC), Mazandaran University of Medical Sciences, Sari, Iran
4 Clinical Research Unit, Mashhad University of Medical Sciences, Mashhad, Iran
5 Immunogenetics Research Center, Mazandaran University of Medical Sciences, Sari, Iran
6 Invasive Fungi Research Center (IFRC), Mazandaran University of Medical Sciences, Sari, Iran
7 Colorectal Research Center, Iran University of Medical Sciences, Tehran, Iran
8 Students Research Committee, Department of Parasitology and Mycology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
doi
10.29252/cmm.3.1.25چکیده
Background and Purpose: Patulin is a mycotoxin produced by some molds,especially Aspergillus and Penicilium, and is responsible for mycotoxicosis in animals and humans.There is still not very detailed data about the anti-cancer potency of patulin, but some reports demonstrated that it induces cellular apoptosis and toxicity. Materials and Methods: To determine the efficacy of patulin as a therapeutic strategy for cervical and colorectal cancers, we investigated its effects on HeLa,SW-48, and MRC-5 cell lines. Cell lines were exposed to various concentrations of patulin (i.e., 0.5, 1, 2, and 4 µM), then using methyl thiazolyl tetrazolium (MTT) and bromo-2′-deoxyuridine (BrdU) assays, the rates of apoptosis and cell viability were determined. Results: The obtained results showed a significant reduction in cell viability and apoptosis induction in a dose-dependent manner. Among all the cell lines, the highest growth inhibition rate was obtained at the 4 μM concentration of patulin.Conclusion: Our results suggested that patulin could significantly decrease tumor growth in human cervical and colorectal cancer models.
