Evaluation of miR-146a expression level in macrophages exposed to Candida glabrata
نویسندگان
1 Department of Medical Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2 Department of Medical Genetics, School of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran
3 Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
4 Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
doi
10.18869/acadpub.cmm.2.2.6چکیده
Background and Purpose: MicroRNAs are small non-coding RNAs with 1924- nucleotides in length. Up- or downregulation of many miRNAs has been shown by stimulation of Toll-like receptors (TLRs) in the innate immune system. Up-regulation of miR-146a has been reported by both TLR and heat-killed Candida albicans. In this study, we aimed to evaluate the expression of miR-146a in cultured monocyte-derived macrophages (MDMs) infected by Candida glabrata at 12, 24, and 48 hours. Materials and Methods: miR-146a expression was evaluated by qRT-real time polymerase chain reaction (PCR) at three time points in C. glabrata-infected MDMs. The data was analyzed using repeated measures ANOVA. Results: miR-146a expression was down-regulated in infected MDMs compared to the control group (p <0.018). The expression of miR-146a was at its highest level at 48 h, as compared to 12 and 24 h (p <0.018). The differences between the experimental group compared to the control group were statistically significant (p <0.018). Conclusion: These results suggest that miR-146a can be involved in regulating macrophage function following TLR stimulation in C. glabrata-infected MDMs.
