Quantitative Assessment of Diosgenin from Different Ecotypes of Iranian Fenugreek (Trigonella foenum-graecum L.) by High-performance Liquid Chromatography

نویسندگان

1 دانشگاه آزاد اسلامی

2 دانشگاه آزاد اسلامی

3 دانشگاه فردوسی مشهد

4

doi
10.22059/ijhst.2018.254988.230
چکیده

In this study, a sensitive, accurate and reproducible high performance liquid chromatographic method was developed for determination of diosgenin from fenugreek ( Trigonella foenum-graecum ) leaves in 22 ecotypes of Iranian fenugreek. The obtained results showed that the highest concentration of diosgenin with 23.8 mg g -1 dw was found in Boshruyeh ecotype. While, Mashhad ecotype with 3.51 mg g -1 dw diosgenin contained the lowest concentration of diosgenin. The method employed in current study confirmed significant variations in the diosgenin concentration in Iranian fenugreek. Therefore, Iranian fenugreek can be considered as a new potential crop and source of diosgenin in the pharmaceutical industry.