PREPARATION AND APPLICATIONS OF FLUORESCENT POLYGLUTARALDEHYDE IMMUNOMICROSPHERES
نویسندگان
doi
چکیده
Aqueous glutaraldehyde has been polymerized under alkaline conditions in the presence of a surfactant to yield microspheres of varied diameters. Microbeads of a nominal 300 nm diameter, made fluorescent via fluoresceinisothiocyanate, were used as a carrier of rabbit anti-human red blood cell antibodies. Specific labeling of the human red blood cells as well as diagnostic use of these fluorescent immunomicrospheresin detection of brucella antibody or antigen has been clearly demonstrated.