فهرست نشریات علمی دسترسی آزاد ایران

Bioethanol is considered the leading biofuel because it is cost-effective in production and meets the increasing global demand for renewable energy. Its production involves effective acid pretreatment or hydrolysis of biomass to convert complex organic material into fermentable sugar. Optimization of substrates, Saccharomyces cerevisiae, and molasses using culture medium, and monitoring bioprocess parameters, was most important in improving yield and efficiency. This research, therefore, aimed at determining various optimal parameters like incubation temperature, different pH levels, and inoculum size, among other optimal conditions, to maximize bioethanol production. Subsequent statistical comparison between the shake flask and 4-liter bioreactor scales was carried out. The results revealed that bioethanol production was higher in the bioreactor scale with a maximum concentration of 16.50 g/L as compared to 4.50 g/L in the shake flask. The productivity was 0.170 g/L/h as compared to the shake flask 3.67 times. The bioethanol yield increased from 0.36 g/g to 1.16 g/g while the biomass yield increased from 0.58 g/g to 1.27 g/g. The result shows bioreactors enhanced efficiency in bioethanol production, hence reduced ecological impact and improved process sustainability. Synthesis further led to increased bioethanol yield and bioresource efficiency through the replacement of raw materials by agricultural byproducts and an improved process.