In vitro Evaluation of the Antiviral Activity and Cytotoxicity Effect of Holothuria leucospilota Sea Cucumber Extracts from the Persian Gulf
نویسندگان
1 Fisheries Department, Natural Resources Faculty, University of Tehran, Karaj, Iran
2 Fisheries Department, Natural Resources Faculty, University of Tehran, Karaj, Iran
3 Persian Gulf & Oman Sea Ecological Research Institute, Bandar Abbas, Hormozgan, Iran
4 Hepatitis & AIDS Departments, Pasteur Institute of Iran, Tehran, Iran
doi
چکیده
Aims: Antiviral activity and effect of methanol and diethyl ether extracts from different parts of sea cucumber (Holothuria leucospilota) against HIV-1 were assessed on human oral epidermoid carcinoma cells (KB) and Human embryonic kidney 293T cells (HEK293T). Materials & Methods: Sea cucumber was collected at a depth of 10-30 m (Persian Gulf). Extracts were prepared by diethyl ether and methanol solvents. The antiviral activity of each extract was evaluated by inhibition of single-cycle HIV-1 (SCR HIV-1) p24 Core antigen production in HeLa cells and cellular toxicity of different extracts were assessed, using a cell proliferation XTT kit. Findings: Antiviral activity of each extract showed that some concentrations were able to inhibit the replication of HIV-1. Diethyl ether extract of body wall with 2.79 TI index displayed the highest antiviral activity as well as less effect. Conclusion: This study showed that crude extracts of Holothuria leucospilota, especially methanol and diethyl ether extracts of digestive organs and body wall and antiviral activity, respectively.
