Evaluation of Total Phenolic Content and Antioxidant Activity in Ten Selected Mahaleb (Prunus mahaleb L.) Genotypes
نویسندگان
1 Department of Agriculture, Shirvan Branch, Islamic Azad University, Shirvan, Iran
2 Department of Agriculture, Shirvan Branch, Islamic Azad University, Shirvan, Iran
3 Metabolic Research Centre, Royal Perth Hospital, School of Medicine and Pharmacology, University of Western Australia, Perth, Australia
4 Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
5 Student Research Committee, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
doi
10.22059/ijhst.2015.56435چکیده
Mahaleb (Prunus mahaleb L.) is an important rootstock for P. avium and P. cerasus cultivars. The present study has compared the phenolic contents and antioxidant activity of the methanolic extracts of the barks, leaves and fruits of ten selected mahaleb genotypes. The total phenolic content (5.11-131.77 mg GA g-1) in barks and the total flavonoid (54.06-180.6 mg QE g-1) and proanthocyanidin (8.89-25.33 mg CA g-1) contents in fruits were greater than the other parts of the plants. The maximum contents of total phenol and total proanthocyanidin were in the stem bark and fruit of the genotype '249' (131.77 mg GA g-1, 25.33 mg CA g-1, respectively), while the maximum contents of flavonoid, and anthocyanin were in the fruits of genotype 271 (180.6 mg QE g-1 and 260.81 mg CY g-1, respectively). Antioxidant activity of the samples was determined using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and reducing power assay (RPA). The antioxidant activity was the highest with the genotype '249', which showed 80.9% and 89.3% in DPPH and RPA assays, respectively. This study showed that total phenolic, flavonoid, proanthocyanidin, and anthocyanin contents were affected by mahaleb genotypes. This information may be of assistance in the production of mahaleb genotypes with maximum levels of desired phenolic.
