An amperometric hand-held Nitrate biosensor based on Nitrate reductase immobilized on a thin film electrode: a tool for in-filed water and food analysis
نویسندگان
1 Bioprocessing and Biodetection Lab (BBL), Department of Food Science, Technology, and Engineering, University of Tehran, Karaj, Iran.
2 Bioprocessing and Biodetection Lab (BBL), Department of Food Science, Technology, and Engineering, University of Tehran, Karaj, Iran
3 Bioprocessing and Biodetection Lab (BBL), Department of Food Science, Technology, and Engineering, University of Tehran, Karaj, Iran.
doi
10.22059/jfabe.2024.373543.1167چکیده
This study presents the design and evaluation of a portable amperometric biosensor for detecting nitrates, utilizing a gold three-electrode system with immobilized nitrate reductase. With a sensitivity of 12.01 μA mM−1 cm−2 over a 0.16–0.96 mM range and a regression coefficient of 0.98, the biosensor outperforms traditional UV spectrophotometric methods (0.01–0.19 mM) by offering a broader range (0.16–0.96 mM) and greater accuracy, as evidenced by a 6.69% relative standard deviation for reproducibility and a detection limit of 0.14 mM. Maintaining 63% of its initial response after four days and showing minimal interference from nitrite (1 mM), this biosensor is highly suitable for water testing, although pre-treatment is required for analyzing food samples due to phenolic compounds.
