Aptamer base nanobiosensor for stress hormone measurement
نویسندگان
1 Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran; Division of Engineering in Medicine, Department of Medicine, Harvard Medical School, Brigham and Women’s Hospital, Cambridge, MA, 02139, USA
2 Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
3 Division of Engineering in Medicine, Department of Medicine, Harvard Medical School, Brigham and Women’s Hospital, Cambridge, MA, 02139, USA
doi
10.52547/jncog.2023.103449چکیده
Cortisol plays an important part in physiological procedures. Observing cortisol concentration as a stress indicator demonstrates the physiological mode for the diagnosis goal. The mechanism of the presented nano biosensor is LSPR and is performed based on the nucleic acid aptamer functionalized with gold nanoparticles which have specific interaction with cortisol. The interaction of aptamer particular measurement of cortisol was accomplished over the concentration from 0.05 -0.2 μg·mL-1 within 20 minutes. The detection limit is estimated to be 0.02 μg.mL-1.
