Neuronal toxicity of biopolymer-template synthesized ZnO nanoparticles
نویسندگان
1 Department of Modern Sciences and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
2 Chemistry Department, Payame Noor University, 19395-4697 Tehran, Iran
3 Department of Modern Sciences and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
4 Nuclear Medicine Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
5 Targeted Drug Delivery Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
doi
10.7508/nmj.2014.02.004چکیده
Objective(s): A simple and "green" method was developed for preparing zinc oxide nanoparticles (ZnO-NPs) in aqueous starch solutions. Starch was used as a stabilizer to control of the mobility of zinc cations and then control growth of ZnO-NPs prepared via a sol-gel method. Because of the special structure of the starch, it permits termination of the particle growth.Materials and Methods: The dried gel was calcined at different temperatures of 400, 500, 600, and 700 °C. The prepared ZnO-NPs were characterized by different techniques such as X-ray diffraction analysis (XRD), transmittance electron microscopy (TEM), and UV-Vis absorption. Results: The XRD results displayed hexagonal (wurtzite) crystalline structure for prepared ZnO nanoparticles with mean sizes below than 50 nm. In vitro cytotoxicity studies on neuro2A cells showed a dose dependent toxicity with non-toxic effect of concentration below 6 μg/mL. Discussion: The results showed that starch is an eco-friendly material that can be used as a stabilizing agent in the sol-gel technique for preparing of ZnO-NPs in a large scale.
