Effect of Solvent Type on Rosmarinic Acid, Total Phenol, Flavonoids, and Antioxidant Activity of Nepeta asterotricha Rech. f: An Endemic Plant from Iran
نویسندگان
1 Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran
2 Department of Biology, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, (Mahan Golsay Company, Yazd) Iran
3 Department of Biology, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran
4 Department of Biology, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran
doi
10.22092/jmpb.2020.351355.1242چکیده
The current study aims to investigate the effect of solvent variety on some phytochemical factors of Nepeta asterotricha Rech. f.: as an endemic plant from Iran. It is suspected that quantity and quality of chemical compounds would be affected by solvent type. For this purpose, aqueous, hydro-alcohol (50/50 ethanol/water), and methanol were used as solvents. The studied factors were total phenol, flavonoid, antioxidant activity, and rosmarinic acid that were measured by Folin-Ciocalteu, colorimetric, FRAP, and HPLC methods, respectively. The outcomes showed hydro-alcohol extraction could significantly isolate phenol compounds (156.84 mg GAE/g DW) with considerable antioxidant properties (318.55 mg AA/g DW). However, methanol was more effective to extract total flavonoid content (101.34 mg RE/g DW) and rosmarinic acid (140.39% DW). In addition, more study should be done to investigate the importance of each compounds for both medicinal and industrial uses.
