فهرست نشریات علمی دسترسی آزاد ایران

Designing a fast, inexpensive, and easy method for on-site detection of pesticide residues is very important. In the present research, a sensitive nanobiosensor has been designed based on gold colloidal nanoparticles that could detect organophosphorus and carbamate pesticides. The biosensor was synthesized using gold plasmonic nanoparticles chemically grown on grain particles and was optimized in the presence of different concentrations and volumes of aptamer, salt, etc. To detect pesticides, this nanobiosensor was used to detect Trichlorfon and diazinon (OP) and carbofuran and pirimicarb (Carb) pesticides were used. Upon interaction with the pesticides, the nanobiosensor changed markedly in appearance and color, which was visible to the naked eye. The results show that the sensitivity of this nanobiosensor is lowest to diazinon and highest to carbofuran over time. In the presence of Trichlorfon, the nanobiosensor initially designed shows significant morphological changes in the nanostructures. The incubation time for pesticide samples with the nanobiosensor is about 10-15 minutes. Another interesting potential of colorimetric nanobiosensors is the extreme sensitivity of surface plasmon resonance in gold nanostructures to the surrounding media, which can be functionalized with specific biomolecules for targeted detection. Due to the ease of synthesis, indigenous production, ease of functionalization, maintenance of biomolecule stability on the surface, and the ability to detect pesticides quickly and visually at the lowest concentrations of analytes, there is strong potential to replace this new method with conventional methods.