How Different Temperatures and Feeding Rates Impact Physiological and Histological Responses of Juvenile Asian Seabass (Lates calcarifer)
نویسندگان
1 Department of Fisheries, Faculty of Nano and Bio Science and Technology, Persian Gulf University, Bushehr, Islamic Republic of Iran.
2 Persian Gulf Research Institute, Persian Gulf University, Bushehr, Islamic Republic of Iran.
3 Department of Fisheries, Faculty of Nano and Bio Science and Technology, Persian Gulf University, Bushehr, Islamic Republic of Iran.
4 Department of Fisheries, Faculty of Nano and Bio Science and Technology, Persian Gulf University, Bushehr, Islamic Republic of Iran.
5 Department of Fisheries, Faculty of Nano and Bio Science and Technology, Persian Gulf University, Bushehr, Islamic Republic of Iran.
6 Persian Gulf Research Institute, Persian Gulf University, Bushehr, Islamic Republic of Iran.
7 Jihoceská univerzita v Ceských Budejovicích, Fakulta rybárství a ochrany vod, Jihoceské výzkumné centrum akvakultury a biodiverzity hydrocenóz, Ústav akvakultury a ochrany vod, Na Sádkách 1780, 370 05 Ceské Budejovice.
doi
10.22034/JAST.27.3.605چکیده
This study evaluated the interactive impacts of water temperature and feeding rate on digestive enzymes, intestine histology, growth and stress-related genes, and cultivable intestinal microbiota of Asian seabass (Lates calcarifer). For this purpose, 180 fish (85.0±3.0 g) were reared at three different temperatures (20, 27, and 33°C) and two feeding rates (apparent satiation and 2.5% of biomass) with three replications for 6 weeks. The results revealed no significant differences among different treatments regarding the activity of digestive enzymes (P˃ 0.05) of fish reared under different temperatures and feeding rates. The length, width, and thickness of intestinal villi were unaffected by different temperatures and feeding rates (P˃ 0.05). In addition, no variations were found in the total aerobic bacterial count of fish gut from different experimental groups (P˃ 0.05). At the molecular level, IGF-I and HSP70 coding genes were found to be highly expressed in experimental treatments (P< 0.05). To conclude, the present study showed that temperatures between 27 to 33°C were more optimal for Asian seabass, and the different temperatures and feeding rates did not affect the digestive enzymes, intestine histology, and gut microbiota of juvenile Asian seabass after 6 weeks.
