Detection and molecular characterization of tomato big bud disease in Qazvin province
نویسندگان
1 Department of Plant Protection, Faculty of Agriculture, Zabol University, Zabol, Iran.
2 Department of Plant Protection, Faculty of Agriculture, Zabol University, Zabol, Iran.
3 Seed and Plant Certification and Registration Institute, AREEO, Karaj, Iran.
4 Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
doi
10.48311/jcp.2019.1443چکیده
Some samples were collected from tomato fields in Qazvin from tomato plants with big bud symptoms such as plant droop and purplish vein under the leaf, enlarged and sac-like pistils and malformed buds. DNA was extracted from the veins and vascular tissues of the plant with CTAB-based methods. In symptomatic plants, DNA fragments of 1800 and 1200bp were amplified by PCR using P1/P7, R16F2n/R16R2 primers. Restriction fragment length polymorphism (RFLP) analysis of nested R16F2n/R16R2 primed PCR product (1200bp) showed that the tomato big bud phytoplasma from Qazvin (TOM-Qazvin) is a member of clover proliferation (16SrVI). Phylogenetic analysis of 16SrRNA and putative restriction site analysis of the R16F2n/R16R2 primed sequence classified TOM phytoplasma in clover proliferation (16SrVI) group and belonged to subgroup 16SrVI-A. Virtual RFLP by using 1200bp sequencing of 16SRNA and 17 restriction enzymes confirmed that TOM-Qazvin belonged to the subgroup 16SrVI-A and16SrVI group. To our knowledge, this is the first report of tomato big bud disease in Qazvin province.
