Metabolic and Enzymatic Responses of Calendula officinalis L. to Foliar Application of Spermidine, Citric Acid and Proline under Drought Stress in a Post Harvest Condition
نویسندگان
1 Department of Horticultural Sciences, Aliabad Katoul Branch, Islamic Azad University, Aliabad katoul, Islamic Republic of Iran.
2 Department of Horticulture Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Islamic Republic of Iran.
3 Department of Horticultural Sciences, Garmsar Branch, Islamic Azad University, Garmsar, Islamic Republic of Iran.
4 Department of Horticultural Sciences, Garmsar Branch, Islamic Azad University, Garmsar, Islamic Republic of Iran.
doi
چکیده
The first experiment was performed to study the effect of foliar application of spermidine, citric acid and proline (0, 50 and 100 mg L-1) on some metabolites and enzymatic activities of Calendula officinalis L. under drought stress (No stress: Control, 25, 50, and 75% field capacity). The second experiment was performed to study the effect of foliar application of spermidine, citric acid and proline (0, 50 and 100 mg L-1) on the post-harvest shelf life of flowers (beginning of the experiment, 5, and 10 days post-harvest). The experiments were factorial in a completely randomized design with 3 replications. All treatments had a significant effect on the measured variables. In the first experiment, FC 25% reduced all traits, FC 75% increased carotenoid, carbohydrate, phenol, flavonoid, protein, Peroxidase (POD) and Superoxide Dismutase (SOD) activities. The highest vitamin C was observed in the control FC. Also, foliar application of 100 mg L-1 proline, increased carotenoid, carbohydrate and phenol, 50 mg L-1 proline increased protein content. Also, 100 mg L-1 of spermidine increased flavonoid and 100 mg L-1 of citric acid increased vitamin C, SOD and POD activities. In the second experiment, all evaluated traits were reduced ten days after harvest, the highest post-harvest life was for 100 mg L-1 of citric acid (9.7 days) and the lowest was for the control (5.3 days). The study results showed that application of 100 mg L-1 spermidine, citric acid and proline with FC 75% improved biochemical properties, nutritional traits, and post-harvest life of Pot marigold.
