Cementum histomorphology after chemical and mechanical root surface treatments for residual periodontal ligament removal

نویسندگان

1 Department of Pediatric Dentistry, School of Dentistry, North Khorasan University of Medical Sciences, Bojnurd, Iran

2 Private Dental Clinic, Mashhad, Iran

3 Department of Pediatric Dentistry, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran

4 Department of Pediatric Dentistry, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran

doi
10.22038/jdmt.2026.93230.1937
چکیده

Objective: Effective removal of necrotic tissue while minimizing cementum damage is essential before replanting avulsed teeth. This study compared different root surface treatments in terms of their effectiveness for removing residual periodontal ligament (PDL) and their impact on histomorphometric properties of cementum.Methods: In this in vitro study, 90 extracted premolars were randomly assigned to nine group based on the root surface treatment applied: Group 1: sterile gauze, Group 2: 5.25% sodium hypochlorite (NaOCl) for 5 minutes, Group 3: 1% NaOCl for 15 minutes, Group 4: 5.25% NaOCl with surfactant for 2 minutes, Group 5: 5.25% NaOCl with surfactant for 5 minutes, Group 6: 1% NaOCl with surfactant for 15 minutes, Group 7: 10% calcium hypochlorite (Ca(OCl)₂) for 35 minutes, Group 8: 10% Ca(OCl)₂ with surfactant for 35 minutes, and Group 9: electric toothbrush. Following treatment, the roots were evaluated histomorphometrically for residual PDL and cementum integrity.Results: Significant differences were observed in residual PDL and cementum integrity among groups (P<0.05). The greatest removal of PDL tissue was achieved with 5.25% NaOCl with surfactant applied for 5 minutes (Group 5) or 2 minutes (Group 4), as well as with 10% Ca(OCl)₂ with surfactant applied for 35 minutes (Group 8) and 10% Ca(OCl)₂ alone (Group 7). Cementum integrity was best preserved in Groups 1, 4, 5, and 6.Conclusions: The addition of surfactant to 5.25% NaOCl improved the removal of residual PDL tissue. Among the protocols, 5.25% NaOCl with surfactant achieved the most effective PDL removal while maintaining optimal cementum integrity.