فهرست نشریات علمی دسترسی آزاد ایران

There is a small population of cells with stem cell properties in cancer tissues that are responsible for the initiation of tumour formation and have the ability to resist apoptosis inducing agents and chemotherapy. SOX2 is a transcription factor in stem cells and OCT4 is a pluripotency factor known as cancer stem cell markers. The use of plant compounds as cancer suppressors has become increasingly important today. The purpose of this study is to investigate the expression of self-renewal genes SOX2 and OCT4 in a prostate cancer cell line (PC3) treated with different concentrations of methanolic, chloroform, aqueous and hexane extracts of the green microalga Haematococcus pluvialis. PC3 cells were exposed to varying concentrations of microalgae extract after cultivation. The percentage of survival was checked using the MTT method, and the change in expression change of SOX2 and OCT4 genes was studied using the RT-qPCR technique.  The results of the evaluations showed that the hexane extract of microalgae caused a significant decrease in the number of living cells compared to the control and significantly decreased the expression of the SOX2 and OCT4 genes. Therefore, the hexane extract of H. pluvialis can be considered a factor affecting the expression pathway in self-renewal genes of stem cells.