Seasonal dynamics of fatty acid composition of Artemia cysts lipids from lakes of Pavlodar Region of Kazakhstan
نویسندگان
1 Laboratory of Hydrobiology, Fisheries Research and Production Centre, Almaty, Kazakhstan
2 Laboratory of Biochemistry, Al-Farabi Kazakh National University, Almaty, Kazakhstan,
3 Laboratory of Hydrobiology, Fisheries Research and Production Centre, Almaty, Kazakhstan
4 Laboratory of Hydrobiology, Director of Aral branch Fisheries Research and Production Centre, Kyzylorda, Kazakhstan
5 Laboratory of Hydrobiology, Fisheries Research and Production Centre, Almaty, Kazakhstan
6 Laboratory of Hydrobiology, Fisheries Research and Production Centre, Almaty, Kazakhstan
doi
10.22124/cjes.2024.7828چکیده
The purpose of this research was to study the fatty acid composition of total lipids of Artemia cysts, Artemia sp., taken from four lakes in the Pavlodar region of Kazakhstan in different seasons. Extraction of total lipids was carried out with a binary mixture of organic solvents chloroform-ethanol (2:1). Gas chromatographic separation of fatty acids was carried out after methanolysis of lipids to obtain methyl esters of fatty acids. Quantification was based on the internal standard method using a calibration curve. The seasonal dynamics in the content of both the main groups of fatty acids and individual fractions was shown since a change in ambient temperature induced a modification of the fatty acid composition of lipids. In particular, by autumn, a decrease in the synthesis of monoenoic acids and an elevation in the amount of saturated fatty acids were established. Alterations in the qualitative composition of saturated fatty acids were also noted, in particular, in the autumn samples. There were no such acids as C6:0 caproic, C8:0 caprylic, C10:0 capric, C11:0 undecanoic, C12:0 lauric, C13:0 tridecanoic.
