Delayed reproductive behavioral responses in Drosophila melanogaster (Diptèra: Drosophilidae) following exposure to aqueous extract of Peganum harmala and the insecticide Spinosad
نویسندگان
1 Ecology Laboratory of Marine and Coastal Environments (EMMAL), Department of Biology, Faculty of Sciences, Badji Mokhtar – Annaba University BP 12, P.O. Box 23000, Annaba, Algeria
2 Ecology Laboratory of Marine and Coastal Environments (EMMAL), Department of Biology, Faculty of Sciences, Badji Mokhtar – Annaba University BP 12, P.O. Box 23000, Annaba, Algeria
3 Ecology Laboratory of Marine and Coastal Environments (EMMAL), Department of Biology, Faculty of Sciences, Badji Mokhtar – Annaba University BP 12, P.O. Box 23000, Annaba, Algeria
4 Ecology Laboratory of Marine and Coastal Environments (EMMAL), Department of Biology, Faculty of Sciences, Badji Mokhtar – Annaba University BP 12, P.O. Box 23000, Annaba, Algeria
5 Ecology Laboratory of Marine and Coastal Environments (EMMAL), Department of Biology, Faculty of Sciences, Badji Mokhtar – Annaba University BP 12, P.O. Box 23000, Annaba, Algeria
6 Genetics, Biotechnology and Bioresources valorization Laboratory Department of Nature and Life Sciences, Faculty of Exact Sciences and Sciences of Nature and Life, Mohamed Kheider University, Biskra, Algeria
7 Ecology Laboratory of Marine and Coastal Environments (EMMAL), Department of Biology, Faculty of Sciences, Badji Mokhtar – Annaba University BP 12, P.O. Box 23000, Annaba, Algeria
8 Ecosystem Diversity and Dynamics of Agricultural Production Systems in Arid Zones Laboratory Department of Agriculture Sciences, Faculty of Exact Sciences and Sciences of Nature and Life, Mohamed Kheider University, Biskra, Algeria
9 Ecology Laboratory of Marine and Coastal Environments (EMMAL), Department of Biology, Faculty of Sciences, Badji Mokhtar – Annaba University BP 12, P.O. Box 23000, Annaba, Algeria
doi
10.22034/jesi.46.2.2چکیده
This study aims to evaluate the delayed effects of two treatments spinosad (a commercially available insecticide) and a natural bioinsecticide (aqueous extract of Peganum harmala) on the sexual behavior and oviposition of vinegar flies (Drosophila melanogaster). Sublethal concentrations (86 mg/ml P. harmala, 0.020 mg/ml Spinosad) of each product were administered via ingestion to second-instar (L2) larvae of D. melanogaster. The results indicated that exposure to Spinosad significantly reduced sexual activity in D. melanogaster, as evidenced by prolonged mating latency and a lower copulation rate. Moreover, its repellent properties alter female oviposition site preference and a marked decrease in the number of eggs laid. In contrast, the natural bioinsecticide (aqueous extract of P. harmala) exerted a milder effect: it reduced the frequency of courtship behaviors; it prevented mating in most individuals. Its moderate repellent effect also influenced oviposition site selection, leading to reduction in egg deposition.
