Effect of gamma irradiation on biochemical properties of Egyptian honeybee venom Apis mellifera lamarckii
نویسندگان
1 1- Molecular Biology Department, National Research Centre, Dokki, Giza, Egypt 2- Proteome Research Laboratory, National Research Centre, Dokki, Giza, Egypt
2 1- Molecular Biology Department, National Research Centre, Dokki, Giza, Egypt 2- Proteome Research Laboratory, National Research Centre, Dokki, Giza, Egypt
3 Natural Products Research Department, National Centre for Radiation Research and Technology, Egyptian Atomic Energy Authority, Egypt
4 Honey Bee Research Department, Plant Protection Research Institute, Agricultural Research Center, Giza, Egypt
doi
10.22034/jesi.46.1.6چکیده
Honey bee venom is a protein-rich secretion whose biochemical properties can be altered when exposed to ionizing radiation. Controlled irradiation has been proposed as a method for reducing allergenicity and improving handling while maintaining core biological characteristics. This study examined how moderate gamma irradiation (4 and 6 kGy) affects total protein concentration and the activities of six enzymatic components phospholipase A₂ (PLA₂), hyaluronidase, superoxide dismutase (SOD), acid phosphatase (ACP), phosphodiesterase (PDE), and acetylcholinesterase (AChE) in venom from Apis mellifera lamarckii. Protein quantification and native PAGE demonstrated a dose–response pattern, with minimal alterations at 4 kGy and more evident reduction at 6 kGy. Enzyme assays revealed a clear biphasic response: activities increased at 4 kGy but declined at 6 kGy. These findings indicate that moderate irradiation can modulate the biochemical activity of venom enzymes in a dose-dependent manner.
