Molecular genetic analysis of the ICE1 gene in Arum korolkowii Regel

نویسندگان

1 Department of Biotechnology, Higher School of Chemical Engineering and Biotechnology, M. Auezov South-Kazakhstan University, Tauke-Khan, Shymkent, 160000, Kazakhstan

2 Department of Biotechnology, Higher School of Chemical Engineering and Biotechnology, M. Auezov South-Kazakhstan University, Tauke-Khan, Shymkent, 160000, Kazakhstan

3 Department of Biotechnology, Higher School of Chemical Engineering and Biotechnology, M. Auezov South-Kazakhstan University, Tauke-Khan, Shymkent, 160000, Kazakhstan

4 International Kazakh-Turkish University named after H.A.Yasavi, Turkestan city

5 South Clinical and Genetic Laboratory, South Kazakhstan Medical Academy, Shymkent, 160000, Kazakhstan

6 Institute of Genetics and Plants Experimental Biology, Academy of Sciences of Uzbekistan, Tashkent Region, Kibray District, Yuqori-Yuz, 111226 Uzbekistan

7 Department of Biotechnology, Higher School of Chemical Engineering and Biotechnology, M. Auezov South-Kazakhstan University, Tauke-Khan, Shymkent, 160000, Kazakhstan

8 Department of Biotechnology, Higher School of Chemical Engineering and Biotechnology, M. Auezov South-Kazakhstan University, Tauke-Khan, Shymkent, 160000, Kazakhstan

9 Institute of Genetics and Plants Experimental Biology, Academy of Sciences of Uzbekistan, Tashkent Region, Kibray District, Yuqori-Yuz, 111226 Uzbekistan

10 South Clinical and Genetic Laboratory, South Kazakhstan Medical Academy, Shymkent, 160000, Kazakhstan

doi
10.22124/cjes.2023.7402
چکیده

The paper presents a molecular genetic analysis of the expression of the ICE 1 gene Arum korolkowii Regel involved in the abiotic stress response in the Korolkov Aronnik in cold and frost conditions. The classical PCR method was used to identify the ICE 1 gene in Aronnik Korolkov and sequenced this site to determine the uniqueness of this gene. When stress is induced, increased expression of the studied gene is shown. It has been proved that the expression of this gene in Aronnik Korolkov begins already at the stage of acclimatization.