Genetic and biological signature of Potato Virus X circulating in Kazakhstan
نویسندگان
1 Institute of Plant Biology and Biotechnology, Almaty, Kazakhstan
2 Institute of Plant Biology and Biotechnology, Almaty, Kazakhstan
3 Institute of Plant Biology and Biotechnology, Almaty, Kazakhstan
4 Saken Seifullin Kazakh Agrotechnical Research University, Astana, Kazakhstan
5 Saken Seifullin Kazakh Agrotechnical Research University, Astana, Kazakhstan
6 Southwest University, Chongqing, China
7 Leling Xisen Potato Industry Group Company Ltd, Leling, China
8 Saken Seifullin Kazakh Agrotechnical Research University, Astana, Kazakhstan
doi
10.22124/cjes.2023.7403چکیده
This research studied ten isolates of potato virus X (PVX) isolated from potato cultivars growing in Kazakhstan (KZ), Belarus (BY) and China (CN). Additionally, the potato samples were screened for Potato virus Y (PVY), Potato virus S (PVS), and Potato leafroll virus (PLRV) using multiplex reverse transcription-polymerase chain reaction (RT-PCR). PVX was isolated from the potato varieties ‘Tamasha’, ‘Red Scarlet’, ‘Gala variety’, ‘Krone’, ‘Juwel’, ‘Gala’, and ‘b2-7’. The identified isolates of PVX were sequenced and used to construct a phylogenetic tree, which demonstrated that KZ and BY isolates form clusters closely related to isolates from Asia and Europe, however, CN isolate formed a separate clade. KZ5 and KZ7 PVX isolates showed local symptoms of mosaic and light green speckled spots on the upper leaves of Datura stramonium in pathogenicity test.
