Optimization the Extraction Efficiency of Phenolic Compounds from Cannabis sativa L. by products using Augmented Simplex-Centroid Design
نویسندگان
1 Laboratory of Functional Ecology and Environmental Engineering, Sidi Mohamed Ben Abdellah University, Faculty of Sciences and Technologies, Department of Biology, P.O. Box 2202 - route d’Imouzzer, Fez, Morocco;
2 Biotechnology, Environmental Technology and Valorization of Bio-Resources Team, Department of Biology, Faculty of Sciences and Techniques Al-Hoceima, Abdelmalek Essaadi University, Tetouan 93000, Morocco
3 Laboratory of Functional Ecology and Environmental Engineering, Sidi Mohamed Ben Abdellah University, Faculty of Sciences and Technologies, Department of Biology, P.O. Box 2202 - route d’Imouzzer, Fez, Morocco;
4 Laboratory of Functional Ecology and Environmental Engineering, Sidi Mohamed Ben Abdellah University, Faculty of Sciences and Technologies, Department of Biology, P.O. Box 2202 - route d’Imouzzer, Fez, Morocco;
5 Laboratory of Applied Organic Chemistry, Sidi Mohamed Ben Abdellah University, Faculty of Sciences and Technologies, Department of Biology, P.O. Box 2202 – route d’Imouzzer, Fez, Morocco
6 Laboratory of Functional Ecology and Environmental Engineering, Sidi Mohamed Ben Abdellah University, Faculty of Sciences and Technologies, Department of Biology, P.O. Box 2202 - route d’Imouzzer, Fez, Morocco
7 Euromed University of Fez (UEMF), Meknes Roed, 3000 Fez, Morocco
8 Laboratory of Functional Ecology and Environmental Engineering, Sidi Mohamed Ben Abdellah University, Faculty of Sciences and Technologies, Department of Biology, P.O. Box 2202 - route d’Imouzzer, Fez, Morocco;
doi
10.22034/crl.2025.494845.1498چکیده
Cannabis sativa L. holds significant potential for treating a range of medical conditions, including chronic pain, epilepsy, multiple sclerosis, and nausea caused by chemotherapy. Ongoing studies continue to uncover new therapeutic uses for cannabis and its derivatives. The present study focused on optimizing the extraction of phenolic compounds and evaluating antioxidant activity from the by-products of three C. sativa varieties-Beldia, Khardala, and Critical-collected from the Taounate region in Morocco. An augmented simplex-centroid design was applied, utilizing a solvent mixture of water, methanol, and ethanol. Total phenolic content (TPC) and antioxidant activity were measured using the DPPH assay, with extractions carried out via sonication. The optimal solvent combinations for maximizing TPC and antioxidant activity were determined for each variety. For Beldia, the most effective mixture-42% water and 58% methanol-yielded a TPC of 30.28 mg GAE/g dry matter (DM) and an IC₅₀ of 0.62 mg/mL. In Khardala, the highest TPC (32.28 mg GAE/g DM) was obtained using 52% ethanol and 48% methanol, while the best antioxidant activity (IC₅₀ = 0.62 mg/mL) was observed with a ternary mixture of 38% water, 32% ethanol, and 30% methanol. For the Critical variety, pure methanol led to the highest TPC (27.64 mg GAE/g DM), whereas a mixture of 40% water, 32% ethanol, and 28% methanol provided the most notable antioxidant activity with an IC₅₀ of 0.88 mg/mL.
