Preservation of Buck Semen Quality During Chilling Storage Using Coenzyme Q10
نویسندگان
1 Animal Science Research Institute of Iran, Agricultural Research Education and Extension Organization, Karaj, Iran.
2 Department of Research, Breeding and Production of Laboratory Animals, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization, Karaj, Iran.
3 Animal Science Research Institute of Iran, Agricultural Research Education and Extension Organization, Karaj, Iran.
4 Animal Science Research Institute of Iran, Agricultural Research Education and Extension Organization, Karaj, Iran.
5 Animal Science Research Institute of Iran, Agricultural Research Education and Extension Organization, Karaj, Iran.
doi
10.32598/ijvm.18.3.1005359چکیده
Background: Storing spermatozoa in a cold environment decreases spermatozoa metabolism while maintaining sperm fertility. Because of their unique physiological characteristics, sperm cells’ reproductive ability in small ruminants is reduced by cooling.Objectives: This study aimed to assess the impact of supplementing chilling medium with coenzyme Q10 (CoQ10) on the quality parameters of buck’s semen after chilling at 4°C.Methods: Semen was collected, diluted, and divided into five groups with different CoQ10 supplementation concentrations (0, 1, 2, 5, and 10 µM CoQ10). Collected semen were stored at 4°C for 50 h. The motility, mitochondrial activity, membrane integrity, viability, and lipid peroxidation were measured after 0, 25, and 50 h of chilling.Results: According to the findings, different concentrations of CoQ10 had no impact (P>0.05) on semen at time 0 of storage. After 25 and 50 h of storage, supplementing the chilling medium with 5 µM CoQ10 increased (P≤0.05) progressive motility (PM), total motility (TM), viability, mitochondrial activity, and membrane integrity compared to other groups. Furthermore, 5 µM CoQ10 produced less lipid peroxidation (P≤0.05) after 25 and 50 h than the other groups.Conclusion: Adding 5 µM CoQ10 to the buck’s semen cooling medium is an effective procedure for protecting the quality of buck spermatozoa during cooling storage.
