Extraction and quantification of epinephrine in human urine based on modified magnetic nanoparticles using fluorescence technique
نویسندگان
1 Department of Chemistry, College of Science, University of Al-Qadisiyah, Iraq
2 Department of Chemistry, College of Science, University of Al-Qadisiyah, Iraq
doi
10.22034/crl.2024.471918.1394چکیده
A simple, highly sensitive, and selective fluorescent method was developed to detect adrenaline, also known as epinephrine (EP). Adrenaline is a crucial hormone, neurotransmitter, and a substance used in drugs and doping. It utilized Fe3O4-APTS (3-aminopropyltriethoxysilane) nanoparticles to enhance the fluorescence of MNPs. Characterization of the synthesized MNPs was conducted through transmission electron microscopy (TEM), field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). Fe3O4 -APTS demonstrated the highest extraction rates of adrenaline from aqueous solutions, synthetic urine, and human urine, with extraction ratios of 99%,96%, and 87% respectively. The detection limit (LOD) and the quantitative limit (LOQ) of adrenaline were determined using fluorescence spectroscopy at room temperature and were found to be 0.038871 μg/L and 0.11779 μg/L. A linear relationship between fluorescence intensity and adrenaline concentrations was established in the range of 4 to 12 μg mL−1. In addition, the experiment achieved approximately 90% disintegration of the bond between Fe3O4-APTS and adrenaline in an aqueous solution. It also achieved 84% disintegration of adrenaline in human urine. Furthermore, the successful application of this method to synthetic and human urine samples highlights its potential for diagnostic purposes
