Evaluation of COI and ITS2 sequences ability to distinguish Lysiphlebus fabarum and Lysiphlebus confusus (Hymenoptera: Aphidiidae)

نویسندگان
Hosseini, R. ID 1
Sohani, M. M. ID 2
Hajizadeh, J. ID 3
Rahimi, S ID 4

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چکیده

All wasp species in the genus Lysiphlebus Förster are solitary endoparasitoids of aphids andconsidered a taxonomically difficult group. In this study a part of COI and ITS2 was used to distinguishtwo closely related species L. fabarum (Marshall) and L. confusus Tremblay & Eady in Iran. Thesequencing with a similarity about 99.5% showed COI's inability to separate the species L. fabarum andL. confusus. The ITS2 region of the rDNA partly separated the species with the similarity of 96.5-98.5%.The result is indicative of DNA barcoding deficiency in the identification of the species, as the two generegions fail to efficiently separate the species.

کلیدواژه‌ها
COI, DNA barcoding, ITS2, Mitochondrial DNA, ribosomal DNA, Lysiphlebus confusus, Lysiphlebus fabarum