Comprehensive analysis of phytochemistry, In Silico toxicity prediction, and antimicrobial efficacy across different fractions of Ammi visnaga L.
نویسندگان
1 Laboratory of Functional Ecology and Environmental Engineering, Faculty of Science and Technology, Sidi Mohamed Ben Abdellah University- Fez, Morocco.
2 OLMANBGPE, Nador Multidisciplinary Faculty, Mohammed 1st University, Oujda, Morocco
3 Laboratory of Natural substances, Pharmacology Environment, Modeling, Health and quality of life. Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, P.O. Box 1796, Fez 30000, Morocco.
4 Laboratory of Functional Ecology and Environmental Engineering, Faculty of Science and Technology, Sidi Mohamed Ben Abdellah University- Fez, Morocco
5 Laboratory of Natural substances, Pharmacology Environment, Modeling, Health and quality of life. Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, Fez, Morocco.
6 Laboratory of Functional Ecology and Environmental Engineering, Faculty of Science and Technology, Sidi Mohamed Ben Abdellah University- Fez, Morocco.
7 Laboratory of Functional Ecology and Environmental Engineering, Department of Biology, Faculty of Science and Technology, Sidi Mohamed Ben Abdellah University, Fes, Morocco
doi
10.22034/crl.2024.429586.1267چکیده
Ammi visnaga L. is a dense source of bioactive compounds with potential antioxidant and antibacterial activities. Different fractions were prepared to determine their total phenolic content and antioxidant activities (TPC, TFC, TAC, and DPPH). While, agar disc diffusion, MIC, and MBC assays were used to examine the antimicrobial effects of different fractions. The phytochemistry of the umbel dry was performed using UHPLC. Aqueous extract registered the highest amounts of TPC, TAC, TFC, and DPPH compared to other extracts (P<0.05). Treatment of antimicrobial results against three bacterial strains (E. coli, P. aeruginosa, and S aureus) and Candida albicans revealed that the aqueous extract exerted interesting activity against all microbes with diameter zones varying from 12 to 17 mm for bacteria and 25 mm for Candida albicans, while the hexane fraction registered the lowest antimicrobial activity against all microbes under study. The umbel contains 16 phenolic compounds whose majority components are isorhamnetin_3-O-rutinoside (50.18%), isorhamnetin_3-O-glucoside (19.35%) and kaempferol_3-O-glucoside (12.04%). Additionally, the in-silico toxicity prediction showed that the main phytochemicals did not possess any toxicity, including hepatotoxicity, carcinogenicity, mutagenicity, and cytotoxicity. The present findings indicate the possible application of Ammi visnaga L. as a useful antimicrobial agent that replaces chemical drugs that induce the emergence of resistance.
