Morphological and Molecular Investigation of Anaplasma Infection in Dromedary Camel (Camelus dromedarius) in Bushehr Province, Iran
نویسندگان
1 Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
2 Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran- Ira
3 Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
4 Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran- Iran
doi
10.22059/ijvm.2020.306095.1005109چکیده
BACKGROUND: Anaplasmosis is caused by an obligate intracellular, gram-negative microorganism, which be-longs to the family Anaplasmatacea and can be transmitted by ticks and other arthropods. OBJECTIVES: The present study aimed to investigate the status of Anaplasma spp. infection by microscopy and molecular methods in dromedary camels in Bushehr province, Iran. METHODS: A total of 139 blood samples were collected from dromedary camels in Bushehr province. Giemsa staining and nested-polymerase chain reaction (PCR) were conducted to detect Anaplasma infection in the drome-dary camels. RESULTS: We found that 27 (19.4%) out of the total 139 blood samples were suspected for the presence of Ana-plasma spp. by morphological study. The PCR and nested-PCR sequencing results showed 111 (80%) and 134 (96%) samples positive for Anaplasma spp. and BLAST search in NCBI GenBank presented 100% identity with Candidatus Anaplasma camelii. CONCLUSIONS: The molecular results presented the high frequency of Candidatus Anaplasma camelii in camels, in Bushehr city.
